Tutorials

An scglue workflow can be divided into the following two stages. We provide separate tutorials for each stage, using the integration of scRNA-seq and scATAC-seq data as an example.

• Stage 1: Data preprocessing
  • Read data
  • Preprocess scRNA-seq data
  • Preprocess scATAC-seq data
  • Construct prior regulatory graph
    • Obtain genomic coordinates
    • Graph construction
  • Save preprocessed data files
• Stage 2: Model training
  • Read preprocessed data
  • Configure data
  • Build and train GLUE model
  • Check integration diagnostics
  • Apply model for cell and feature embedding